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Estimation of the concentrations of cells, astaxanthin and glucose in a culture ofPhaffia rhodozymaby near infrared reflectance spectroscopy

✍ Scribed by Yuichi Yamane; Takashi Mikami; Katsuya Higashida; Toshihide Kakizono; Naomichi Nishio

Publisher: Springer-Verlag
Year: 1996
Tongue: English
Weight: 349 KB
Volume: 10
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00159519

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✦ Synopsis

Near infrared reflectance spectroscopy (NIR) was employed to estimate the concentrations of cells, astaxanthin and glucose in the culture broth of Phzj'ia rhodozymz. The culture broth (119 samples) was directly subjected for NIR analysis without any pretreatment. When the data obtained by NIR were compared with those obtained by conventional methods, high correlation coefficients were obtained: 0.9S for cells, 0.99 for astaxanthin and 0.94 for glucose. These results suggest that NIR analysis, which is very simple and requires only 3 to 5 min for a sample, is applicable to monitor P. rhodozyma cultures.

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