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Estimation of submicrogram quantities of protein using the dye eosin Y

✍ Scribed by A.A Waheed; P.D Gupta

Book ID
117331582
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
228 KB
Volume
42
Category
Article
ISSN
0165-022X

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Eosin B and eosin Y have been used to estimate micro- and submicrogram quantities of proteins respectively as shown in our previous reports. In the present study we describe the mechanism of eosin binding to proteins. At pH lower than 3.0 the absorbance of unbound dye is greatly reduced. After the d