Estimation of Protein Using Eosin B Dye

✍ Scribed by A.A. Waheed; P.D. Gupta

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 85 KB
• Volume: 233
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1996.0037

✦ Synopsis

249

the fact that the viscosity of no-gel sieving medium bp number of the DNA fragment. Figure 2 indicates that the plots of migration time vs bp number of DNA frag-decreases with the increase in the Joule heating effect when the concentration of sodium chloride is increased. ment were nonlinear shapes. The cubic spline function expresses the relationship between migration time and Meanwhile, the resolution of DNA decreases with an increase in the concentration of sodium chloride, due bp number. to the Joule heating effect.

Acknowledgment. This research was supported by the Postdoctor Foundation of China.

Reproducibility, linearity, and detection limits. Reproducibility is important for qualitative and quantitative analyses of DNA fragments. The RSDs of migra-REFERENCES tion times and peak area of DNA fragments in two 1.