Estimation of state and amount of phenylalanine residues in proteins by second derivative spectrophotometry
β Scribed by Tetsuo Ichikawa; Hiroshi Terada
- Book ID
- 113126128
- Publisher
- Elsevier Science
- Year
- 1979
- Weight
- 556 KB
- Volume
- 580
- Category
- Article
- ISSN
- 0005-2795
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π SIMILAR VOLUMES
A method for deconvolution of the near-uv second-derivative spectra of proteins into their component tryptophan, tyrosine, and phenylalanine spectra is described. In this approach, the second-derivative spectra of tryptophan and tyrosine model compounds are numerically shifted to create a set of ref
The mutual interference between the second-derivative bands of tyrosine and tryptophan in proteins has been evaluated in terms of the ratio r between two peak-to-peak distances. The r values have been found to be well related, although not linearly, to the tyrosine/ tryptophan ratio in both model co