Estimation of FAD-monooxygenase in microsomal preparations

✍ Scribed by Joy Cavagnaro; Elmer J. Rauckman; Gerald M. Rosen

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 658 KB
• Volume: 118
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90180-9

✦ Synopsis

The determination of the mixed function flavin-containing monooxygenase of liver by NADPH oxidation or oxygen uptake is complicated by a significant endogenous rate in the absence of specific substrate. We have defined optimal conditions for measuring this enzyme activity in hepatic microsomal preparations using NADPH oxidation. We have also found that if substrate stimulated minus endogenous NADPH oxidation rates are measured, this enzyme will be underestimated. Data are also presented which suggest that this endogenous rate can be at least partly accounted for by the presence of an endogenous substrate.