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Estimation of binding sites from the adsorption profile of complexed DNA

โœ Scribed by Subhendu Ghosh; Anjali Mookerjee

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
575 KB
Volume
20
Category
Article
ISSN
0020-7608

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โœฆ Synopsis

Abstract

The adsorption profiles of free and Daunomycin bound DNA at an aluminaโ€“H~2~O interface have been studied. Proper experimental conditions were maintained to considerably reduce intercalation and to make electrostatic binding predominant in the DNAโ€“Daunomycin complex. For both free and complexed DNA, Langmuir plots gave straight lines. Adsorption of drug bound DNA was less than that of free DNA. This has been explained from the viewpoint of electrostatic interactions between adsorbate and adsorbent. A Langmuirโ€type model for adsorption of polymers combined with Scatchard equation have been used to estimate the average value of percent of phosphates in DNA stacked with Daunomycin.

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Fluorescence of proflavineโ€“DNA complexes
Fluorescence of proflavineโ€“DNA complexes: Heterogeneity of binding sites
โœ J. C. Thomes; G. Weill; M. Daune ๐Ÿ“‚ Article ๐Ÿ“… 1969 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 634 KB

Measurements of the relative quantum yield of fluorescence of proflavine bound to DNA as a function of the number of bound dyes per nucleotide and the ionic strength allow the determination of the binding constants and respective number of the two types of sites previously postulated. It is demonstr