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Establishment of cell lines derived from the rat suprachiasmatic nucleus

✍ Scribed by Soshi Kawaguchi; Atsuki Shinozaki; Masuo Obinata; Kaoru Saigo; Yoshiyuki Sakaki; Hajime Tei


Book ID
116294461
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
463 KB
Volume
355
Category
Article
ISSN
0006-291X

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Primary cultured cells from the presumptive anlage of the rat suprachiasmatic nucleus (SCN) were immortalized by infection with a retroviral vector encoding the adenovirus 12S E1A gene. After drug selection, the resulting neural cell lines (SCN1.4 and SCN2.2) displayed (a) extended growth potential