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Establishment of a practical enzymatic assay method for determination of isovaleryl-CoA dehydrogenase activity using high-performance liquid chromatography

✍ Scribed by Go Tajima; Nobuo Sakura; Hiroko Yofune; Andi Dwi Bahagia Febriani; Yutaka Nishimura; Akiko Sakamoto; Hiroaki Ono; Yosuke Shigematsu; Masao Kobayashi

Book ID
116350056
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
232 KB
Volume
353
Category
Article
ISSN
0009-8981

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A Highly Sensitive High-Performance Liqu
A Highly Sensitive High-Performance Liquid Chromatography– Fluorometric Method for the Assay of Peptidylarginine Deiminase Activity
✍ Toshiyuki Chikuma; Michiyuki Yamada; Ayumi Tsuda; Masaru Yamamoto; Katsuhiko Nak πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 68 KB

The activity of peptidylarginine deiminase (PAD) has generally been assayed by a colorimetric method using N-benzoyl-L-arginine ethyl ester (BAEE) and Nbenzoyl-L-arginine (Bz-L-Arg) as the substrates. The widespread occurrence of citrulline and urea in tissues makes use of this method difficult, esp