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Establishment and characterization of human B-lymphocytic lymphoma cell lines (balm-3, -4 and -5): Intraclonal variation in the B-cell differentiation stage

✍ Scribed by Ming-Swang Lok; Hirofumi Koshiba; Tin Han; Syuiti Abe; Jun Minowada; Avery A. Sandberg

Publisher
John Wiley and Sons
Year
1979
Tongue
French
Weight
788 KB
Volume
24
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

This study describes the establishment of three non‐Burkitt B‐lymphoma cell lines (BALM‐3, BALM‐4 and BALM‐5) originating from the pleural effusion of a patient with a poorly differentiated diffuse lymphocytic lymphoma. The cells of BALM‐3, ‐4 and ‐5 exhibited a number of properties which distinguish them from the usual B‐cell type lymphoblastoid cell lines. Thus, they lacked the Epstein‐Barr virus genome and had abnormal chromosome constitutions including a 14q+ marker. The presence of the identical surface immunoglobulin isotypes (γ and χ chain determinants), and la‐like B‐cell‐associated antigen in the cultured cells and in the “fresh” lymphoma cells in vivo was demonstrated. These findings strongly suggested that these cell lines have B‐cell characteristics and were derived from the original tumor cell population. BALM‐5 cells, however, showed somewhat different growth, cell surface marker profile and functional characteristics compared to those of BALM‐3, and ‐4 cells. These variations suggest that the BALM‐5 cells were probably at different stages of B‐cell maturation than those of BALM‐3 and ‐4, even though all three cell lines (established in three separate flasks) originated from the cells of the same pleural effusion of a lymphoma with monoclonal B‐cell characteristics.

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