Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity
β Scribed by Mulligan, Martin E.; Hawley, Diane K.; Entriken, Robert; McClure, William R.
- Book ID
- 111982353
- Publisher
- Oxford University Press
- Year
- 1984
- Tongue
- English
- Weight
- 480 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0305-1048
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Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained. The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the beta-subunit of RNA poly
A cold-sensitive mutation in the rpoB gene for the RNA polymerase beta subunit increasing the temperature of promoter opening on T2 phage DNA was obtained in Escherichia coli. The mutation also affects the stages preceding promoter opening by increasing the dissociation rate of RNA polymerase--DNA c
Escherichia coli cells, carrying a rifampicin sensitive RNA polymerase beta-subunit gene in the chromosome and a rifampicin resistant beta-subunit gene placed under the control of a strong promoter in a multicopy plasmid, are unable to grow in the presence of rifampicin, despite the accumulation of