A New Data Analysis Method to Determine
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Michelle Arkin; J.D. Lear
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Article
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2001
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Elsevier Science
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English
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In principle, equilibrium analytical ultracentrifugation (AU) can be used to quantify the binding stoichiometry and affinity between small-molecule ligands and proteins in aqueous solution. We show here that heteromeric binding constants can be determined using a data-fitting procedure which utilize