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Equilibrium ultracentrifugation of polymers: A new method of data reduction with an application to TMV protein

โœ Scribed by Charles L. Stevens


Book ID
115905207
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
303 KB
Volume
56
Category
Article
ISSN
0014-5793

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In principle, equilibrium analytical ultracentrifugation (AU) can be used to quantify the binding stoichiometry and affinity between small-molecule ligands and proteins in aqueous solution. We show here that heteromeric binding constants can be determined using a data-fitting procedure which utilize