A New Data Analysis Method to Determine Binding Constants of Small Molecules to Proteins Using Equilibrium Analytical Ultracentrifugation with Absorption Optics: Volume 299, Number 1 (2001), pages 98–107

A New Data Analysis Method to Determine

A New Data Analysis Method to Determine Binding Constants of Small Molecules to Proteins Using Equilibrium Analytical Ultracentrifugation with Absorption Optics

✍ Michelle Arkin; J.D. Lear 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 154 KB

In principle, equilibrium analytical ultracentrifugation (AU) can be used to quantify the binding stoichiometry and affinity between small-molecule ligands and proteins in aqueous solution. We show here that heteromeric binding constants can be determined using a data-fitting procedure which utilize