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Equilibrium studies on neutral red–DNA binding

✍ Scribed by Frederick G. Walz Jr.; Barry Terenna; David Rolince

Publisher
Wiley (John Wiley & Sons)
Year
1975
Tongue
English
Weight
640 KB
Volume
14
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Spectrophotometric binding studies were undertaken on the interaction of neutral red with native and heat‐denatured, sonicated, calf thymus DNA in a 0.2__M__ ionic strength buffer containing Tris–sodium acetate–potassium chloride at 25°C. The p__K__~A~ of neutral red was found to be 6.81. At pH 5 the binding of protonated neutral red was complicated even at low concentration ratios of dye to DNA. In the pH range 7.5–8.5 the tight binding process could be studied and it was found that both protonated and free base species of neutral red significantly bind with DNA having association constants (in terms of polynucleotide phosphate) of 5.99 × 10^3^ M^−1^ and 0.136 × 10^3^ M^−1^, respectively, for native DNA and 7.48 × 10^3^ M^−1^ and 0.938 × 10^3^ M^−1^, respectively, for denatured DNA. The p__K__~A~ value of the neutral red–DNA complexes were 8.46 for native DNA and 7.72 for denatured DNA. These results are discussed in terms of possible binding mechanisms.

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