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Equilibrium dissociation and unfolding of the arc repressor dimer

✍ Scribed by Bowie, James U.; Sauer, Robert T.

Book ID
120477194
Publisher
American Chemical Society
Year
1989
Tongue
English
Weight
555 KB
Volume
28
Category
Article
ISSN
0006-2960

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## Abstract Conditions for in vitro unfolding and refolding of dimeric thymidylate synthase from __Lactobacillus casei__ were found. Ultraviolet difference and circular dichroism spectra showed that the enzyme was completely unfolded at concentrations of urea over 5.5 M. As measured by restoration