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Dissociation and Unfolding of Insulin Dimers

✍ Scribed by Ganim, Ziad; Jones, Kevin C.; Tokmakoff, Andrei

Book ID
123157837
Publisher
Biophysical Society
Year
2009
Tongue
English
Weight
39 KB
Volume
96
Category
Article
ISSN
0006-3495

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## Abstract Conditions for in vitro unfolding and refolding of dimeric thymidylate synthase from __Lactobacillus casei__ were found. Ultraviolet difference and circular dichroism spectra showed that the enzyme was completely unfolded at concentrations of urea over 5.5 M. As measured by restoration