Epstein-Barr virus: Transformation of lymphocytes separated by size or exposed to bromodeoxyuridine and light
β Scribed by E. Henderson; J. Robinson; A. Frank; G. Miller
- Book ID
- 119009374
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 873 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0042-6822
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A method of producing human monoclonal antibody by combining somatic cell hybridization technology with the capability of Epstein-Barr virus (EBV) to transform human B lymphocytes is described. Peripheral blood lymphocytes from a donor with positive tuberculin skin test reaction were transformed by
## Abstract The mechanism of the previously observed enhancement of EpsteinβBarr virus (EBV)βinduced cell transformation of human lymphocytes by 12β__O__βtetradecanoylβphorbolβ13βacetate (TPA) was studied. A concentration of TPA (0.5 ng/ml) was used. In cultures of human cord blood lymphocytes infe