Generation of human monoclonal autoantibody-producing cell lines by epstein-barr virus transformation of autoreactive B lymphocytes and by somatic cell hybridization techniques: Application to the analysis of the autoimmune B cell repertoire
β Scribed by Minoru Nakamura; Paolo Casali
- Book ID
- 119587681
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 767 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1058-6687
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method of producing human monoclonal antibody by combining somatic cell hybridization technology with the capability of Epstein-Barr virus (EBV) to transform human B lymphocytes is described. Peripheral blood lymphocytes from a donor with positive tuberculin skin test reaction were transformed by
## Abstract Four EpsteinβBarr virusβpositive lymphoblastoid cell lines (LCL) were successfully infected __in vitro__ with immunodeficiency virus type 1 (HIVβ1) as demonstrated by reverse transcriptase activity and p24 HIV antigen in culture supernatants, positive cell staining for gagβencoded HIV p