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Epstein-Barr virus-induced cap formation in human lymphoblastoid cells

✍ Scribed by Yorio Hinuma; Masahiro Suzuki; Takeshi Sairenji

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
419 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Redistribution and consequent cap formation of Epstein‐Barr virus adsorbed to human lymphoblastoid cells were studied by indirect membrane immunofluorescence carried out at 0° C. When EBV was adsorbed on cells at 0° C, the cell sur face fluorescence had a mostly ring‐like pattern. However, the ring cells could be transformed into cap cells when warmed at 37° C. This cap formation could be induced by EBV alone without participation of antibodies involved in the immunofluorescence procedure. The cap formation was temperature‐ and pH‐dependent, and was reversibly inhibited by sodium azide or some sugars. Thus the EBV‐induced cap formation was analogous to that induced by antibodies or ligands on other lymphoid cells.

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