Epstein-Barr-encoded RNA in situ hybridization: Diagnostic applications
β Scribed by Richard F. Ambinder; Risa B. Mann
- Book ID
- 116083578
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 602 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1532-8392
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Epstein-Barr virus (EBV)-encoded RNAs (EBERs) are the most abundant viral transcripts in latently EBV-infected cells. Recently, we found that EBERs play a key role in the maintenance of malignant phenotypes of Burkitt's lymphoma (BL) cells. They confer clonability in soft agarose, tumorigenicity in
We have developed a procedure for quantitative assay of Epstein-Barr virus (EBV)-infected cells in suspension in either latent or replicative phase using in situ hybridization and flow cytometry. The cells were hybridized with EBV-specific digoxigenin or biotin-labeled oligonucleotide probes, follow