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Epigenetic silencing of PEG3 gene expression in human glioma cell lines

✍ Scribed by Shinji Maegawa; Hirotaka Yoshioka; Noriko Itaba; Norika Kubota; Shigeki Nishihara; Yasuaki Shirayoshi; Eiji Nanba; Mitsuo Oshimura

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
192 KB
Volume
31
Category
Article
ISSN
0899-1987

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✦ Synopsis

Abstract

Genomic imprinting, the phenomenon in which alleles of genes are expressed differentially depending on their parental origins, has important consequences for mammalian development, and disturbance of normal imprinting leads to abnormal embryogenesis and some inherited diseases and is also associated with various cancers. In the context of screening for novel imprinted genes on human chromosome 19q13.4 with mouse A9 hybrids, we identified a maternal allele–specific methylated CpG island in exon 1 of paternally expressed imprinted gene 3 (PEG3), a gene that exhibits paternal allele–specific expression. Because PEG3 expression is downregulated in some gliomas and glioma cell lines, despite high‐level expression in normal brain tissues, we investigated whether the loss of PEG3 expression is related to epigenetic modifications involving DNA methylation. We found monoallelic expression of PEG3 in all normal brain tissues examined and five of nine glioma cell lines that had both unmethylated and methylated alleles; the remaining four glioma cell lines exhibited gain of imprinting with hypermethylated alleles. In addition, treatment of glioma cell lines with the DNA demethylating agent 5‐aza‐2′‐deoxycytidine reversed the silencing of PEG3 biallelically. In this article, we report that the epigenetic silencing of PEG3 expression in glioma cell lines depends on aberrant DNA methylation of an exonic CpG island, suggesting that PEG3 contributes to glioma carcinogenesis in certain cases. © 2001 Wiley‐Liss, Inc.

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