𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Regulation of DNA repair gene expression in human cancer cell lines

✍ Scribed by Claire J. McGurk; Michele Cummings; Beate Köberle; John A. Hartley; R. Timothy Oliver; John R. Masters

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
349 KB
Volume
97
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Although most advanced cancers are incurable, the majority of testicular germ cell tumors can be cured using cisplatin‐based combination chemotherapy. The nucleotide excision repair (NER) pathway removes most DNA adducts produced by cisplatin, and the low levels of NER in testis tumor cells may explain why these cancers are curable. Three NER proteins: ERCC1, XPF, and XPA, are present at low levels in testis tumor cell lines, and addition of these proteins to protein extracts of testis tumor cells increases their in vitro DNA repair capacity to normal levels. The aim of this study was to identify the mechanism responsible for the low levels of these DNA repair proteins. The levels of the mRNA transcripts for ERCC1, XPF, and XPA were measured in a panel of 14 different human cancer cell lines, using real‐time PCR. Three ERCC1 splice variants were identified and quantitated. Three alternative transcription start points (TSPs) were identified for ERCC1 but none were testis‐specific. The significantly lower levels of ERCC1, XPF, and XPA protein in testis tumor cell lines cannot be explained solely by differences in transcriptional efficiency or mRNA stability. For ERCC1, post‐transcriptional control by alternative splicing does not account for the testis‐specific low levels of protein expression. Pulse‐chase experiments showed that the half‐life of ERCC1 protein in a testis tumor cell line was not significantly different to that in a prostate cancer cell line. Taken together, these results suggest that constitutive levels of these DNA repair proteins are controlled at the level of translation. J. Cell. Biochem. 97: 1121–1136, 2006. © 2005 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Expression of somatic DNA repair genes i
Expression of somatic DNA repair genes in human testes
✍ Danuta Galetzka; Eva Weis; Nicolai Kohlschmidt; Oliver Bitz; Raimund Stein; Thom 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB

## Abstract Meiosis is the key process for recombination and reduction of the diploid chromosome set to a haploid one. Many genes that have been found in yeast or mouse models to play a role in meiosis are also important for the repair of DNA damage in somatic cells. To study the DNA repair gene tr

Construction and characterization of mul
Construction and characterization of multiple human colon cancer cell lines for inducibly regulated gene expression
✍ Arkadiusz Welman; Christopher Cawthorne; Jane Barraclough; Nigel Smith; Gareth J 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 379 KB

## Abstract Validation of targets for cancer drug discovery requires robust experimental models. Systems based on inducible gene expression are well suited to this purpose but are difficult to establish in several epithelial cell types. Using the recently discovered transcriptional transactivator (

Expression and regulation of the meprin
Expression and regulation of the meprin β gene in human cancer cells
✍ Gail L. Matters; Judith S. Bond 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 246 KB 👁 1 views

A novel mRNA isoform (meprin b H ) of the cell-surface protease subunit meprin b was previously identified in human colon cancer cells. The study reported here revealed that this mRNA isoform was identical within the protein coding region and at the 3 H end to the b isoform of normal intestine but t

Regulation of u-PA gene expression in hu
Regulation of u-PA gene expression in human prostate cancer
✍ Christopher P. Evans; Eschelle C. Stapp; Marc A. Dall'Era; Jose Juarez; Joy C. Y 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 151 KB 👁 2 views

u-PA contributes to CaP progression, especially in the metastatic androgen-insensitive state. In vitro, u-PA is expressed by androgen-insensitive, but not androgen-sensitive, CaP cell lines. We hypothesized that in androgen-sensitive CaP an activated ARE represses u-PA expression but in androgen-ins

Role of MED1 (MBD4) Gene in DNA repair a
Role of MED1 (MBD4) Gene in DNA repair and human cancer
✍ Alfonso Bellacosa 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 230 KB

## Abstract The human protein MED1, also known as MBD4, was isolated in a yeast two‐hybrid screening as an interactor of the mismatch repair protein MLH1. MED1 contains an N‐terminal 5‐methylcytosine binding domain (MBD), which allows binding to methylated DNA, and a C‐terminal catalytic domain wit

Transcriptional regulation of urokinase
Transcriptional regulation of urokinase (uPA) gene expression in breast cancer cells: Role of DNA methylation.
✍ Rosie H. Xing; Shafaat A. Rabbani 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 156 KB 👁 2 views

Carcinoma of the breast is a leading hormone-dependent malignancy, resulting in a high rate of morbidity and mortality. During the complex multi-step process of tumor promotion, this common cancer is initiated as hormone-responsive (HR), non-metastatic cancer, followed by a gradual transition into a