Epidermal growth factor binding and trafficking dynamics in fibroblasts: relationship to cell proliferation

✍ Scribed by Cindy Starbuck; H.Steven Wiley; Douglas A. Lauffenburger

• Book ID: 103008696
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 597 KB
• Volume: 45
• Category: Article
• ISSN: 0009-2509
• DOI: 10.1016/0009-2509(90)80117-w

✦ Synopsis

We provide a mathematical model, with accompanying quantitative experimental data, for binding and trafficking properties of the epidermal growth factor (EGF) receptor on B82 fibroblasts, and propose a theoretical dependence of cell proliferation rate on these properties.

The signal for cell proliferation is generated by intrinsic EGF-receptor (EGFR) tyrosine kinase activation via EGF binding at the cell surface, and terminated by receptor/growth factor complex internalization and degradation.

Our model consists of kinetic equations which describe the binding, internalization, and recycling of EGF and EGFR, along with a simple expression relating the dependence of cell cycle progression on EGFP dynamics.

We show that, with key model parameters determined independently from EGF/fibroblast binding and internalization experiments, our model successfully predicts, as a fist step, kinetic data for EGF binding to and internalization by B82 cells at 37'C.