Enzymology at the Membrane Interface: Intramembrane Proteases

✍ Scribed by Michael H. Gelb (Eds.)

Publisher: Academic Press
Year: 2017
Leaves: 474
Series: Methods in Enzymology Volume 584
Edition: 1st Edition
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Enzymology at the Membrane Interface: Intramembrane Proteases, Volume 584, the latest release in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

✦ Table of Contents

Content:
Series PagePage ii
CopyrightPage iv
ContributorsPages xi-xiii
PrefacePages xv-xixMichael H. Gelb
Chapter One - Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P ProteasePages 1-33Y. Hizukuri, K. Akiyama, Y. Akiyama
Chapter Two - Signal Peptidase Enzymology and Substrate Specificity ProfilingPages 35-57R.E. Dalbey, D. Pei, Ö.D. Ekici
Chapter Three - Screening and Characterization Strategies for Nanobodies Targeting Membrane ProteinsPages 59-97S. Veugelen, M. Dewilde, B. De Strooper, L. Chávez-Gutiérrez
Chapter Four - Probing the Activity of Eukaryotic Rhomboid Proteases In VitroPages 99-126B. Cordier, M.K. Lemberg
Chapter Five - Expression, Purification, and Enzymatic Characterization of Intramembrane ProteasesPages 127-155R. Zhou, Y. Shi, G. Yang
Chapter Six - Analyzing Amyloid-β Peptide Modulation Profiles and Binding Sites of γ-Secretase ModulatorsPages 157-183J. Trambauer, A. Fukumori, B. Kretner, H. Steiner
Chapter Seven - Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility MethodPages 185-205T. Tomita
Chapter Eight - A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman SpectroscopyPages 207-228J.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi, I. Ubarretxena-Belandia
Chapter Nine - An Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the MembranePages 229-253R.P. Baker, S. Urban
Chapter Ten - Production of Recombinant Rhomboid ProteasesPages 255-278E. Arutyunova, R. Panigrahi, K. Strisovsky, M.J. Lemieux
Chapter Eleven - Mechanism and Inhibition of Rhomboid ProteasesPages 279-293K. Strisovsky
Chapter Twelve - Enzymatic Assays for Studying Intramembrane ProteolysisPages 295-308D.M. Bolduc, D.J. Selkoe, M.S. Wolfe
Chapter Thirteen - Methods for Structural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA SuperfamilyPages 309-347Y. Yang, N. Ke, S. Liu, W. Li
Chapter Fourteen - Functional Study of the Vitamin K Cycle Enzymes in Live CellsPages 349-394J.-K. Tie, D.W. Stafford
Chapter Fifteen - Activity Assays for Rhomboid ProteasesPages 395-437E. Arutyunova, K. Strisovsky, M.J. Lemieux
Author IndexPages 439-464
Subject IndexPages 465-474

✦ Subjects

Home;Books & Journals;Biochemistry, Genetics and Molecular Biology;Biochemistry;Enzymology;Enzymology at the Membrane Interface: Intramembrane Proteases

📜 SIMILAR VOLUMES

Enzymology at the Membrane Interface: In

📁 Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

✍ Michael H. Gelb (Eds.) 📂 Library 📅 2017 🏛 Academic Press

</header><div itemprop="description" class="collapsable text"><P><EM>Enzymology at the Membrane Interface</EM>, the latest volume in the <EM>Methods in Enzymology </EM>series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzy

Particles at Fluid Interfaces and Membra

📁 Particles at Fluid Interfaces and Membranes

✍ K. Nagayama 📂 Library 📅 2001 🏛 Elsevier Science 🌐 English

In the small world of micrometer to nanometer scale many natural and industrial processes include attachment of colloid particles (solid spheres, liquid droplets, gas bubbles or protein macromolecules) to fluid interfaces and their confinement in liquid films. This may lead to the appearance of late

Particles at Fluid Interfaces and Membra

📁 Particles at Fluid Interfaces and Membranes

✍ P. Kralchevsky, K. Nagayama 📂 Library 📅 2001 🏛 Elsevier Science 🌐 English

Membranes and Membrane Processes

📁 Membranes and Membrane Processes

✍ Ryohei Hori, Ken-ichi Inui, Mikihisa Takano (auth.), Enrico Drioli, Masayuki Nak 📂 Library 📅 1986 🏛 Springer US 🌐 English

<p>During the past two decades Membrane Science and Technology has made tremendous progress and has changed from a simple laboratory tool to large scale processes with numerous applications in Medicine and Industry. In this volume are collected papers presented at the First Europe Japan Congress on

Protein–Membrane Interactions

📁 Protein–Membrane Interactions

✍ Joseph F. Hoffman and Gerhard Giebisch (Eds.) 📂 Library 📅 1990 🏛 Elsevier, Academic Press 🌐 English

Membrane Protein-Cytoskeleton Interactio

📁 Membrane Protein-Cytoskeleton Interactions

✍ W. James Nelson (Eds.) 📂 Library 📅 1996 🏛 Academic Press 🌐 English

The plasma membrane acts as both a boundary and a site of exchange between the outside and the inside of a cell. The cytoskeleton plays essential roles in delineating membrane protein distributions, in defining structural and functional domains in the plasma membrane, and in regulating membrane prot