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Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

✍ Scribed by Michael H. Gelb (Eds.)

Publisher
Academic Press
Year
2017
Leaves
409
Series
Methods in Enzymology Volume 583
Edition
1st Edition
Category
Library
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✦ Synopsis

Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

✦ Table of Contents

Content:
Series PagePage ii
CopyrightPage iv
ContributorsPages xi-xiv
PrefacePages xv-xixMichael H. Gelb
Chapter One - A High-Throughput Fluorometric Assay for Lipid–Protein BindingPages 1-18W. Cho, Y. Hu, K. Baek, H. Kim
Chapter Two - Fluorescence-Based In Situ Quantitative Imaging for Cellular LipidsPages 19-33W. Cho, Y. Yoon, S.-L. Liu, K. Baek, R. Sheng
Chapter Three - Preparation of the Full Set of Recombinant Mouse- and Human-Secreted Phospholipases A2Pages 35-69F. Ghomashchi, V. Brglez, C. Payré, L. Jeammet, S. Bezzine, M.H. Gelb, G. Lambeau
Chapter Four - Cellular Assays for Evaluating Calcium-Dependent Translocation of cPLA2α to MembranePages 71-99B. Yun, C.C. Leslie
Chapter Five - Secreted Phospholipase A2 Specificity on Natural Membrane PhospholipidsPages 101-117K. Yamamoto, Y. Miki, H. Sato, R. Murase, Y. Taketomi, M. Murakami
Chapter Six - Analyses of Calcium-Independent Phospholipase A2beta (iPLA2β) in Biological SystemsPages 119-141S.E. Barbour, S. Ramanadham
Chapter Seven - Using Hydrogen–Deuterium Exchange Mass Spectrometry to Examine Protein–Membrane InteractionsPages 143-172O. Vadas, M.L. Jenkins, G.L. Dornan, J.E. Burke
Chapter Eight - Reversible Chemical Dimerization by rCD1Pages 173-195M. Schifferer, S. Feng, F. Stein, C. Schultz
Chapter Nine - Interfacial Enzymes: Membrane Binding, Orientation, Membrane Insertion, and ActivityPages 197-230S.A. Tatulian
Chapter Ten - Measuring Diacylglycerol Kinase-θ Activity and BindingPages 231-253B. Tu-Sekine, D.M. Raben
Chapter Eleven - Studying Gastric Lipase Adsorption Onto Phospholipid Monolayers by Surface Tensiometry, Ellipsometry, and Atomic Force MicroscopyPages 255-278A. Bénarouche, L. Sams, C. Bourlieu, V. Vié, V. Point, J.F. Cavalier, F. Carrière
Chapter Twelve - Probing Conformational Changes and Interfacial Recognition Site of Lipases With Surfactants and InhibitorsPages 279-307E. Mateos-Diaz, S. Amara, A. Roussel, S. Longhi, C. Cambillau, F. Carrière
Chapter Thirteen - Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging MethodsPages 309-325F. Philip, E.E. Ha, M.A. Seeliger, M.A. Frohman
Chapter Fourteen - PLD Protein–Protein Interactions With Signaling Molecules and Modulation by PAPages 327-357J. Gomez-Cambronero, A.J. Morris, K.M. Henkels
Chapter Fifteen - Analysis of Phosphatidic Acid Binding and Regulation of PIPKI In Vitro and in Intact CellsPages 359-374L.W.R. Tay, Z. Wang, G. Du
Author IndexPages 375-397
Subject IndexPages 399-409

✦ Subjects

Home;Books & Journals;Biochemistry, Genetics and Molecular Biology;Biochemistry;Enzymology;Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding

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