✦ LIBER ✦

Enzymic oxidation of dopamine by polyphenol oxidaze

✍ Scribed by T. C. Tan; Y. Chen

Book ID: 102927971
Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 515 KB
Volume: 24
Category: Article
ISSN: 0538-8066
DOI: 10.1002/kin.550241112

No coin nor oath required. For personal study only.

✦ Synopsis

The kinetics of the enzymic oxidation of dopamine (A) by polyphenol oxidaze (E) was studied by spectrophotometrically monitoring the concentration of the reaction product, 2,3-dihydroindole-5,6 quinone, at 470 nm. The kinetics model proposed for the reaction and the effect of pH agreed with the experimental data and the resulting rate equation is given by

where [A] is the concentration of dopamine (mM), [E,] the concentration of polyphenol oxidaze (mg/l) and [H+] the concentration of hydrogen ion (M) in the reaction mixture.

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