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Enzymic activity and conformational properties of native and crosslinked glucose oxidase

โœ Scribed by Beca Solomon; Noah Lotan; Ephraim Katchalski-Katzir

Publisher
Wiley (John Wiley & Sons)
Year
1977
Tongue
English
Weight
782 KB
Volume
16
Category
Article
ISSN
0006-3525

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โœฆ Synopsis

Abstract

Glucose oxidase has been modified by reacting it with glutardialdehyde. The products obtained are water soluble and merely intramolecularly crosslinked; they exhibit high enzymic activity and good stability towards denaturing agents. Comparative circular dichroism studies have been performed with the native and crosslinked enzymes, as well as with the corresponding apoenzymes. The results suggest that the FAD coenzyme is not a gross structural determinant of glucose oxidase.

๐Ÿ“œ SIMILAR VOLUMES

Comparative fluorescence studies of nati
Comparative fluorescence studies of native and crosslinked glucose oxidase
โœ Beca Solomon; Meir Shinitzky ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 444 KB

## Abstract The binding characteristics of flavin adenine dinucleotide (FAD) to apoenzyme preparations obtained from native and intramolecularly crosslinked glucose oxidase were determined and compared. The dissociation constants __K__~diss~ as well as rates of recombination of FAD with the two apo