Enzyme-linked immunosorbent assay (ELISA) using a glycolipid antigen for the serodiagnosis of melioidosis
β Scribed by Le Van Phung; Yuki Han; Shiro Oka; Hisako Hotta; Michael D. Smith; Prapit Theeparakun; Eiko Yabuuchi; Ikuya Yano
- Book ID
- 109307141
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 551 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0928-8244
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Recombinant Japanese encephalitis (JE) virus proteins were evaluated as antigens for serodiagnosis of JE using an enzyme-linked immunosorbent assay (ELISA). The premembranel membrane (prMiM) and envelope (E) proteins of JE virus were expressed in HeLa cells infected with a recombinant vaccinia virus
We have developed an ELISA for IgM and IgG antibodies to the core glycolipid (CGL) of the Re mutant Salmonella minnesota R 595, and to lipid A. Anti-CGL antibodies have been detected in sera from 37% of healthy blood donors, whereas anti-lipid A activities were found in 13% of individuals only. The