A direct enzyme-linked immunosorbent assay (ELISA) for antibodies to enterobacterial Re core glycolipid and lipid A

✍ Scribed by M. Nys; P. Damas; F. Damas; L. Joassin; J. Demonty

• Book ID: 104657228
• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 688 KB
• Volume: 176
• Category: Article
• ISSN: 0300-8584
• DOI: 10.1007/bf00190532

✦ Synopsis

We have developed an ELISA for IgM and IgG antibodies to the core glycolipid (CGL) of the Re mutant Salmonella minnesota R 595, and to lipid A. Anti-CGL antibodies have been detected in sera from 37% of healthy blood donors, whereas anti-lipid A activities were found in 13% of individuals only. The anti-CGL and anti-lipid A activities were examined in patients in a surgical intensive care unit, selected on the basis of a definite risk of infectious complications due to Gram-negative bacteria. Of the patients who developed such infections, the rate of favourable outcome was significantly higher in patients with either stable positive or increasing anti-CGL activities than in patients found to be negative. Our results provide clear evidence that anti-CGL antibodies contribute to host defence against various Gram-negative bacteria.