Enzyme-linked immunosorbent assay (ELISA) for the determination of the toxic glycoside cleistanthin B
β Scribed by G. Ragupathi; P. Prabhasankar; P. Chandra Sekharan; K.S. Annapoorani; C. Damodaran
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 646 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0379-0738
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β¦ Synopsis
Cleistanthus collinus is a highly toxic plant frequently implicated in suicidal and homicidal poisoning cases referred to our laboratory. An enzyme-linked immunosorbent assay (ELISA) is reported in this paper for the quantitation of the active principles of C. collinus. This method is highly reproducible and sensitive to detect as low as 2 ng/ml of cleistanthin B, a toxic constituent of C. collinus. The ELISA can be successfully applied for the specific identification of C. collinus poisoning in clinical and forensic toxicology.
π SIMILAR VOLUMES
An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymer