The use of enzyme-linked immunosorbent assays (ELISA) for the determination of Triton X nonionic detergents

✍ Scribed by Siong I. Wie; Bruce D. Hammock

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 707 KB
• Volume: 125
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90399-2

✦ Synopsis

An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-permilliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures.