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Enzyme-linked immunoassay (ELISA) for connective tissue components

✍ Scribed by S.I. Rennard; R. Berg; G.R. Martin; J.M. Foidart; P.Gehron Robey


Book ID
102983226
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
671 KB
Volume
104
Category
Article
ISSN
0003-2697

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✦ Synopsis


RIOCHEMIST-RY 104. 205-214 (1980) ' Abbreviations used: ELISA. enzyme-linked immunoadsorbant assays; HRP, horseradish peroxidase: PBS. 20 mM sodium phosphate. 0.15 ~1 sodium chloride.


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## Abstract An ELISA for the detection of mumps virus‐specific IgG and IgM antibodies was developed. Three different antigen preparations were compared. Equally good results were obtained with virus concentrated by ultracentrifugation and with virus that was further purified by sucrose gradient cen