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Enzyme kinetics — The steady-state observed

✍ Scribed by Paul D Buckley; Leonard F Blackwell; Michael F Dunn; Jeremy P Hill


Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
187 KB
Volume
18
Category
Article
ISSN
0307-4412

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Attempts to apply traditional techniques of enzyme kinetic analysis (Lineweaver-Burk and Eadie-Hofstee plots) to enzymes compartmentalized by the membranes of cells, organelles, or vesicular membrane fragments will generally lead to incorrect estimates of the kinetic constants of the enzymes and inc

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The theory of the steady-state kinetics of irreversible enzyme-catalyzed homopolpmerization and copolymerization on primers has been developed. The rate law for homopolymerization is of the Michaelis-Menten form, but the kinetic parameters depend on primer concentration. Copolymerization has been tr