An operational definition of the steady state in enzyme kinetics
β Scribed by EA Barnsley
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 180 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0307-4412
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π SIMILAR VOLUMES
Attempts to apply traditional techniques of enzyme kinetic analysis (Lineweaver-Burk and Eadie-Hofstee plots) to enzymes compartmentalized by the membranes of cells, organelles, or vesicular membrane fragments will generally lead to incorrect estimates of the kinetic constants of the enzymes and inc
A power series solution is presented which describes the steady-state concentration profiles for substrate and product molecules in immobilized enzyme systems. Diffusional effects and product inhibition are incorporated into this model. The kinetic consequences of diffusion limitation and product in