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Enzyme kinetics far from the standard quasi-steady-state and equilibrium approximations

✍ Scribed by S. Schnell; P.K. Maini

Publisher: Elsevier Science
Year: 2002
Tongue: English
Weight: 532 KB
Volume: 35
Category: Article
ISSN: 0895-7177
DOI: 10.1016/s0895-7177(01)00156-x

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✦ Synopsis

Analytic

approximations of the time-evolution of the single enzyme-substrate reaction are valid for all but a small region of parameter spsce in the positive initial enzyme-initial substrate concentration plane. We find velocity equations for the substrate decomposition and product formation with the aid of the total quasi-steady-state approximation and an aggregation technique for cases where neither the more normally employed standard nor reverse quasi-steady-state approximations are valid. Applications to determining reaction kinetic parameters are discussed.

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