a-Human atrial natriuretic polypeptide (a-was 30-90 fg (10-30 attomo1es)ltube and hANP) in plasma was directly measured 0.6-2.3 ng (0.2-0.75 pmol)/L using 0.04without extraction by sensitive sandwich 0.05 ml of plasma. The lower detection enzyme immunoassay. Polystyrene balls limit was obtained usin
Enzyme immunoassay for α-human atrial natriuretic polypeptide — direct measurement of plasma level
✍ Scribed by Seiichi Hashida; Eiji Ishikawa; Kazuwa Nakao; Masashi Mukoyama; Hiroo Imura
- Book ID
- 115826026
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 455 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0009-8981
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A novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for a-human atrial natriuretic peptide (a-hANP) in plasma, which uses only one monoclonal IgG for the ring structure of a-hANP, is described. Plasma was filtered through polysaccharide membrane to separate p
A novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for a- human atrial natriuretic peptide (a-hANP) in plasma is described. a-hANP was biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-a-hANP [6-281 IgG-coated polys