Direct measurement of α-human atrial natriuretic polypeptide in plasma by sensitive enzyme immunoassay

✍ Scribed by Seiichi Hashida; Eiji Ishikawa; Masashi Mukoyama; Kazuwa Nakao; Hiroo Imura

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 634 KB
• Volume: 2
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860020308

✦ Synopsis

a-Human atrial natriuretic polypeptide (a-was 30-90 fg (10-30 attomo1es)ltube and hANP) in plasma was directly measured 0.6-2.3 ng (0.2-0.75 pmol)/L using 0.04without extraction by sensitive sandwich 0.05 ml of plasma. The lower detection enzyme immunoassay. Polystyrene balls limit was obtained using monoclonal antiwere coated with monoclonal anti-a-hANP a-hANP (ring) IgG,. Plasma hANP levels (ring) lgG, specific for the N-terminal half of healthy men in a supine position after of the ring structure including Met-12 resian overnight fast were 24.5 f 13.2 (SD) due of a-hANP or monoclonal anti-a-hANP nglL and tended to decrease (15.3 f 8.5 (N-terminus) lgG, specific for the N-termi-[SD] ng/L) after intravenous administration nus of a-hANP. Rabbit anti-a-hANP (C-ter-of furosemide and subsequent one hour minus) Fab' specific for the C-terminus of walking. The present sandwich enzyme a-hANP was conjugated to horseradish immunoassay could be modified further to peroxidase. The polystyrene ball was in-improve the detection limit of plasma 01- cubated with a-hANP standards or plasma hANP (0.2 nglL) or to perform a less timeand, after washing, with the conjugate, and consuming one-step sandwich enzyme imbound peroxidase activity was assayed by munoassay without much loss of the fluorimetry (two-step sandwich enzyme im-sensitivity. munoassay). The detection limit of a-hANP