𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Enzyme immobilization on palmityl–sepharose

✍ Scribed by Mohsen Nemat-Gorgani; Khashayar Karimian

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
644 KB
Volume
25
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Enzymes adsorbed on palmityl-substituted Sepharose 4B by hydrophobic interactions have been used in reactor-type experiments. Results presented on immobilized glutamate dehydrogenase, trypsin, a-chymotrypsin, and amyloglucosidase indicate possible potential of the method for continuous catalytic operations. Glutamate dehydrogenase used as a model allosteric enzyme was found to retain its allosteric properties after binding to the absorbent in the form of column or suspension. Thermal stabilities of glutamate dehydrogenase and a-chymotrypsin were significantly decreased upon adsorption, while that of trypsin was apparently unaltered. Results are discussed in terms of specific interactions involving palmityl residues present on the matrix. Relevance of these observations to in vivo processes are also discussed.

📜 SIMILAR VOLUMES

Immobilization of human intestinal mucos
Immobilization of human intestinal mucosa enzymes on sepharose
✍ P. Würsch 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 485 KB

Digestive enzymes from human intestinal mucosa were solubilized by Triton X-100 and papain and covalently bound to cyanogen bromide-activated Sepharose 4-B gel. Triton X-100 solubilized most of the activities, and 39.1 to 63.5% were immobilized on the carrier. The other enzymes, still bound on the m

Immobilization of invertase on sepharose
Immobilization of invertase on sepharose-linked enzyme glycosyl recognizing polyclonal antibodies
✍ Farahdiba Jafri; M. Saleemuddin 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 105 KB

Polyclonal antibodies directed against the yeast invertase glycosyls were raised by immunizing rabbits with neoglycoprotein-I and neoglycoprotein-II. The neoglycoproteins were prepared by separately coupling the N-linked large and small molecular weight yeast invertase oligosaccharides respectively

Enzyme immobilization on fibrin
Enzyme immobilization on fibrin
✍ J. G. Dillon; C. W. R. Wade; W. H. Daly 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 301 KB 👁 1 views
Enzyme immobilization on heparin
Enzyme immobilization on heparin
✍ Torchilin, V. P. ;Il'Ina, E. V. ;Streltsova, Z. A. ;Smirnov, V. N. ;Chazov, E. I 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 271 KB

## Abstract We describe the preparation and some of the properties of heparin‐bound α‐chymotrypsin that were obtained via activation of heparin with water‐soluble carbodiimide. Immobilized enzyme has unchanged kinetic characteristics toward low‐molecular‐weight and macromolecular substrates. The he

Enzyme Immobilization on Porous Silicon
Enzyme Immobilization on Porous Silicon Surfaces
✍ S. E. Létant; B. R. Hart; S. R. Kane; M. Z. Hadi; S. J. Shields; J. G. Reynolds 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 379 KB 👁 2 views

**β‐Glucuronidase enzymes** have been attached to a porous silicon surface through a direct silicon–carbon bond based linking system (see Figure). The attached enzymes display high activity and the photoluminescent (PL) properties and surface stability of the porous silicon are retained. Quenching o