Control of building-block assembly and phase behavior is crucial for the ultimate design of functional architectures ranging from the nano-to the macroscales, with organogels representing one important example of this functional architecture. [1] An ideal gelator is an amphiphile that induces gel formation through self-assembly into highly ordered structures in which the hydrophilic moieties interact through extensive hydrogen bonding and the hydrophobic moieties interact with the organic liquid. Among the growing list of low molecular weight compounds that induce gelation, [2] sugars appear to satisfy these requirements, and numerous alkyl-and aryl-based monosaccharide gelators have been generated. [3] As opposed to chemical synthesis, enzymatic catalysis is highly selective and has been used to generate low-molecularweight compounds that can gel organic solvents, [4] generate a wide range of sugar-based esters, [5] and in particular, prepare highly regioselective symmetrical diesters. [6] We reasoned that such a biocatalytic approach would provide an alternative route to the synthesis of disaccharide-based diesters with physical and structural features appropriate for a lowmolecular-weight gelator along with a controlled symmetry that may aid in self-assembly. Combining the principles of supramolecular chemistry with the selectivity of biocatalysis may represent a new and powerful strategy to develop new molecularly defined and functional materials.