Enzymatic synthesis of l-[4-13C]aspartic acid
β Scribed by Hidekatsu Maeda; Shu-Ichi Suzuki; Masanao Ikeguchi; Tsuyoshi Sakai; Kunihiko Shibata
- Book ID
- 114398853
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 463 KB
- Volume
- 89
- Category
- Article
- ISSN
- 1389-1723
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## Abstract We have developed a stereoselective route to isotopically labeled Lβaspartic acid using Lβserine as a chiral precursor. Labeled serine, prepared biosynthetically was Nβprotected by conversion to the Nβ__t__βBoc derivative. (Nβ__t__βBoc)β[3β^13^C]Serine is cyclized to its Ξ²βlactone which
A synthesis of the title compound from /13Clparaformaldehyde and [15N)ammonium chloride V f 8 ethyl 2-(1 ,3-/2-13Cldithianyl) acetate 3a is described. Ethyl/3-13C13-oxopropanoate derived in sl'tu from 3a was converted by a stepwise Strecker procedure to DL-[ 2-13C,15Nlaspartic acid 7a.
An improved h i g h y i e l d , r a c e m i z a t i o n f r e e s y n t h e s i s o f 85 % ' 3C(U) -L -A s p a r a g i n e by 6c a r b o x a m i d e f o r m a t i o n on e n r i c h e d L -A s p a r t i c a c i d i s r e p o r t e d .