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Enzymatic synthesis of isotopically labeled isoprenoid diphosphates

✍ Scribed by Dale J. Christensen; C.Dale Poulter

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 826 KB
Volume: 2
Category: Article
ISSN: 0968-0896
DOI: 10.1016/0968-0896(94)85011-9

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✦ Synopsis

Recombinant yeast isopentenyl diphosphatc (IPP) isomerase and aviau famesyl diphosphate (FPP) synthase from overproducing strains of Escherichiu coli were used to synthesize FFP from IPP and dimeth '%.JIPP and [2,4,5-%,]DMAFP were synthesized from ethyl [2-13C]bromoacetate and lallyl diphosphate (DMAFP). [2,4,5-[1,3-Y 3C2]ace.tone. These compounds were used as substrates for enzymatic synthesis of FPP selectively labeled at the first or third isoprene residue or at all three.

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