Enzymatic synthesis of functional oligosaccharides as prebiotics by the use of glycosidases
โ Scribed by Wei Li; Xiaoli Xiang; Shufen Tang; Yuhong Tang; Yi Sun; Xiaoxiong Zeng
- Book ID
- 118473491
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 74 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0168-1656
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๐ SIMILAR VOLUMES
Syntheses of oligosaccharides" using isolated glycosidases[" or glycosyl transferdse~,~~] or a combination of both,L4] have been studied extensively during the last decade. A general limitation of this methodology is the low availability and the high cost of glycosyl transferases, though some glycos
&Galactosidmeactivitiesfromthe recombinanttbermophilicCLONEZYMEmglycosidase librarywers screenedat70"C for catalysisof atrarrsgalactosylation &omo-nitrophenyl$galactopyranosideto N-aeetylglucosamine.Threethermophilicglycosidases(GlyOO1-06, -07 and -09) were foundto producepredominantlythe ~(1-4)-lin
During lactose conversion at 70ยฐC, when catalyzed by โค-glycosidases from the archea Sulfolobus solfataricus (SsโคGly) and Pyrococcus furiosus (CelB), galactosyl transfer to acceptors other than water competes efficiently with complete hydrolysis of substrate. This process leads to transient formation