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Chemical and Enzymatic Synthesis of Glycoconjugates 2. High Yielding Regioselective Synthesis of N-Acetyllactosamine by Use of Recombinant Thermophilic Glycosidases Library

✍ Scribed by Jun Li; Peng G. Wang

Publisher: Elsevier Science
Year: 1997
Tongue: French
Weight: 509 KB
Volume: 38
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(97)10141-1

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✦ Synopsis

&Galactosidmeactivitiesfromthe recombinanttbermophilicCLONEZYMEmglycosidase librarywers screenedat70"C for catalysisof atrarrsgalactosylation &omo-nitrophenyl$galactopyranosideto N-aeetylglucosamine.Threethermophilicglycosidases(GlyOO1-06, -07 and -09) were foundto producepredominantlythe ~(1-4)-linkedisomer,Gal ~(1-4)GlcNAcwith up to 610/0 yield and less than 10Yo of the hydrolysisside reactionproduct. Thus, commercialrecombinantthermophilic enzymelibrariesconstitutea novelclass of biocatalystsfor preparativeorganicsynthesis. 01997 Elsevier Science Ltd.

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