𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Enzymatic preparation of high specific activity radiolabeled (I)-l-5-methyltetrahydropteroylglutamate

✍ Scribed by Donald W. Horne; William T. Briggs; Conrad Wagner

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
615 KB
Volume
83
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

5-CH,[G-3H]H,RteGlu

(sp act 5.0 CL'mmol) was synthesized by direct enzymatic reduction of [G-3H]PteGlu by dihydropteroylglutamate reductase to H,RteGlu. The latter was reacted with formaldehyde to give S,lO-CH,-H,-RteGlu which was reduced to the final product by sodium borohydride. A single chromatographic step on TEAE-cellulose afforded S-CH,[G-3H]H,PteGlu in high yield at a radiochemical purity of about 99%.

πŸ“œ SIMILAR VOLUMES

Preparation of 5-fluorouracil-6-3H of hi
Preparation of 5-fluorouracil-6-3H of high specific activity
✍ JΓ­Ε™Γ­ Filip; FrantiΕ‘ek VyΕ‘ata πŸ“‚ Article πŸ“… 1969 πŸ› John Wiley and Sons 🌐 French βš– 448 KB

Isotopic exchange of hydrogen in a system of s~lvent-water-~H was used to prepare 5-Juoroorotic a ~i d -~H ( G ) which was decarboxylated and the hydrogen-3H removed from labile bonds to yield 5'-Juorouracil-6-3H. It was found in tracer experiments that a suitable solvent for the isotopic exchange o