✦ LIBER ✦

Enzymatic hydrolysis of desoxyribonucleic acid prepared with the use of strong sodium hydroxide

✍ Scribed by Charles A. Zittle

Publisher: Elsevier Science
Year: 1948
Tongue: English
Weight: 160 KB
Volume: 245
Category: Article
ISSN: 0016-0032
DOI: 10.1016/0016-0032(48)90840-0

No coin nor oath required. For personal study only.

✦ Synopsis

Hydroxide.--CHARLES A. ZITTLE. It was previously reported (1) that desoxyribonucleic acid (DNA) prepared under mild conditions (method of Hammarsten) (2) was readily hydrolyzed by specific nuclease so that it became soluble in HC1, whereas DNA prepared with the use of hot 1.25 N NaOH (method of Levene) (3) was not hydrolyzed by the nuclease. Further study has shown that under the proper conditions the alkali-treated DNA can be slowly hydrolyzed by the nuclease at a rate about 20 per cent. of that of DNA (Hammarsten). The previously reported .erroneus conclusion that DNA (Levene) could not be hydrolyzed was in consequence of this low rate of hydrolysis and the instability of the enzyme under the conditions used.

📜 SIMILAR VOLUMES

Effect of specific nuclease and phosphoe

Effect of specific nuclease and phosphoesterase on desoxyribonucleic acid prepared with the use of strong sodium hydroxide (method of levene)

✍ Charles A. Zittle 📂 Article 📅 1947 🏛 Elsevier Science 🌐 English ⚖ 174 KB

## Effect of Specific Nuclease and Phosphoesterase on Desoxyribonucleic Acid Prepared with the Use of Strong Sodium Hydroxide (Method of Levene ).--C~IARLES A. ZITTLE. Desoxyribonucleic acid prepared under mild conditions is readily hydrolyzed by specific nuclease so that it becomes soluble in HCI

Effect of peracetic acid, sodium hydroxi

Effect of peracetic acid, sodium hydroxide and phosphoric acid on cellulosic materials as a pretreatment for enzymatic hydrolysis

✍ Mohamed A. Farid; Hossam M. Shaker; Ahmed I. El-Diwany 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 307 KB

The preparation of immobilized lactase a

The preparation of immobilized lactase and its use in the enzymatic hydrolysis of acid whey

✍ Howard H. Weetall; Noshir B. Havewala; Wayne H. Pitcher Jr.; Clarence C. Detar; 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 736 KB

The preparation of crystalline polypropy

The preparation of crystalline polypropylene sodium sulfonates and their interaction with an included sodium hydroxide phase

✍ T. Huff; B. H. Johnson 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 520 KB

Study on the curing behavior of resol. I

Study on the curing behavior of resol. IV. Comparison of the curing behavior of resols prepared with either sodium hydroxide or barium hydroxide

✍ Akinori Fukuda; Kiichi Hasegawa; Hikaru Horiuchi 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 407 KB 👁 1 views

NMR spectroscopy analysis of oligoguluro

NMR spectroscopy analysis of oligoguluronates and oligomannuronates prepared by acid or enzymatic hydrolysis of homopolymeric blocks of alginic acid. Application to the determination of the substrate specificity of Haliotis tuberculata alginate lyase

✍ Alain Heyraud; Claude Gey; Christine Leonard; Cyril Rochas; Sylvie Girond; Berna 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 594 KB

The 1H and 13C NMR chemicals shifts of the various saturated and unsaturated timers obtained by acid or enzymatic depolymerisation of homopolymeric blocks of alginates are reported. In addition, 13C NMR chemical shifts are assigned for several saturated oligomers of higher polymerisation degrees. Br