Effect of specific nuclease and phosphoesterase on desoxyribonucleic acid prepared with the use of strong sodium hydroxide (method of levene)

✍ Scribed by Charles A. Zittle

• Publisher: Elsevier Science
• Year: 1947
• Tongue: English
• Weight: 174 KB
• Volume: 243
• Category: Article
• ISSN: 0016-0032
• DOI: 10.1016/0016-0032(47)90154-3

✦ Synopsis

Effect of Specific Nuclease and Phosphoesterase on Desoxyribonucleic Acid Prepared with the Use of Strong Sodium Hydroxide (Method of Levene

).--C~IARLES A. ZITTLE. Desoxyribonucleic acid prepared under mild conditions is readily hydrolyzed by specific nuclease so that it becomes soluble in HCI. By subsequent action of phosphoesterase from calf intestinal mucosa the nucleic acid was found to be completely hydrolyzed to nucleosides and phosphoric acid (1). Evidence is presented herein that desoxyribonucleic acid prepared by the method of Levene (2), which involves the use of hot 1.25 N NaOH, is not acted on by the nuclease and is only slowly, although extensively, acted on by the phosphoesterase. Evidence is also presented that the resistance of this nucleic acid to the nuclease concerns the adenine radical.