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Enzymatic hydrolysis of cellulose by the cellobiohydrolase domain of CelB from the hyperthermophilic bacterium Caldicellulosiruptor saccharolyticus

โœ Scribed by Joshua I. Park; Michael S. Kent; Supratim Datta; Bradley M. Holmes; Zhaohua Huang; Blake A. Simmons; Kenneth L. Sale; Rajat Sapra


Book ID
108071437
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
590 KB
Volume
102
Category
Article
ISSN
0960-8524

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Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time cou