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Enzymatic determination of the branched-chain α-keto acids

✍ Scribed by G.W. Goodwin; M.J. Kuntz; R. Paxton; R.A. Harris

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
350 KB
Volume
162
Category
Article
ISSN
0003-2697

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✦ Synopsis

A spectrophotometric endpoint assay for determination of branched-chain cu-keto acids is described. The assay depends on measurement of the NADH produced after addition of branched-chain cY-keto acid dehydrogenase. Interference by pyruvate and cy-ketobutyrate was eliminated by pretreating the sample with pyruvate dehydrogenase. The method yielded a peripheral venous plasma value of 59 + 5 JIM (mean + SE) for the branchedchain a-keto acids of five overnight fasted healthy humans. Q 1987 Academic press Inc.

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