✦ LIBER ✦

Enzymatic determination of betaine in rat tissues

✍ Scribed by John J. Martin; James D. Finkelstein

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 412 KB
Volume: 111
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90230-x

No coin nor oath required. For personal study only.

✦ Synopsis

We have developed a sensitive and specific assay for the content of betaine in animal tissues. The method depends on the methylation of homocysteine by YCH,]betaine in the presence of the enzyme betaine:homocysteine methyltransferase. Dilution of the radioactive substrate by the betaine contained in processed extracts reduces the specific activity of the products. Since the reciprocal of product radioactivity is a linear function of the amount of betaine in this sample, the latter can be determined. This compound may be synthesized from [*4C]methyl PCA, perchloric acid.

iodide and N, N-dimethylglycine (10).

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