Determination of cystathionine in rat tissues using isotachophoresis

✍ Scribed by Hiroyuki Kodama; Noriko Mizoguchi; Keiko Sasaki; Hiroaki Mikasa

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 252 KB
• Volume: 133
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90227-0

✦ Synopsis

A method for measurement of cystatbionine in biological samples has been developed by using an isotachophoretic analyzer. The determination of the amount of cystathionine was carried out by measuring a zone length of cystathionine in isotachophoresis. The amount of cystathionine in brains of normal rats determined by using this method was 0.084 + 0.023 pmol/g. This value agreed well with earlier reports. The amount of cystathionine in rats with experimental cystathioninuria was determined in several tissues. The results determined by using tbis method for the determination of cystathionine in the rat tissues agreed well with the results obtained by using an amino acid analyzer.