Enzymatic cross-linking of proteins with tyrosinase
✍ Scribed by Claudia Thalmann; Thomas Lötzbeyer
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 83 KB
- Volume
- 214
- Category
- Article
- ISSN
- 0044-3026
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Attempts were made to cross-link several collagenous proteins to fibronectin with Factor XIIIa (plasma transglutaminase). Cross-linking was demonstrated with type I collagen, type II collagen, type III collagen, type V or AB collagen, and alpha 1(I)-CB7 and alpha 1(I)-CB8 cyanogen bromide fragments
## Abstract The enzyme peroxidase, a substrate (hydrogen donor), and hydrogen peroxide aggregated and polymerized soluble proteins included in the reaction mixture. Gel filtration and acrylamide disk gel electrophoresis revealed newly formed dimers, trimers, and higher protein polymers. Some of the